各位老师:
今天下午1:00在闵行校区实验楼B楼报告厅307有美国院士的精彩报告,请大家踊跃参加并通知感兴趣的研究生前往听报告!

黄静


ABSTRACT

East China Normal University
October 29, 2007

Transcriptional Activation Mechanisms in Animal Cells

Robert G. Roeder
The Rockefeller University

Eukaryoyic protein-coding genes are transcribed by RNA polymerase II, in  conjunction with general initiation and elongation factors, in response to various DNA-binding regulatory factors. Various biochemical and genetic analyses have implicated a variety of coactivators in transcriptional activation by these DNA-binding proteins.  These coactivators include both chromatin remodeling/histone modifying factors (including various histone acetyltransferases and methyltransferases) and factors (such as the 30-subunit Mediator complex) that facilitate direct communication between promoter-bound activators and the general transcription machinery.  We have employed biochemically defined cell free systems reconstituted with recombinant chromatin templates and purified factors to identify and to analyze both independent and cooperative functions of cofactors, as well as underlying mechanisms.  The function of Mediator and selected histone modifying factors will be discussed in relation to gene activation by tumor sup
pressor p53 and by nuclear receptors.