讲座题目:Supercomplex, an Emerging Mechanism for Mitochondrial Respiration
主讲人:白益东 美国德克萨斯大学San Antonio健康科学中心副教授
主持人: 翁杰敏 教授
时间:2013-09-16 10:30-11:30 （周一）
报告人简介：Dr. Yidong Bai（白益东）, 1985年本科毕业于复旦大学微生物学专业，1996年获得美国哥伦比亚大学生物科学博士学位，之后在美国加州理工学院（Caltech, Pasadena, CA）做博士后研究，2001年到美国德克萨斯大学San Antonio健康科学中心工作，目前任该中心副教授。
报告摘要：The primary function of mitochondrial function is to generate ATP through oxidative phosphorylation (OXPHOS). The composition of OXPHOS system has been fully illustrated, and structures of all individual complexes have been solved. However the organization and, in particular, the dynamics of this bioenergetics machinery has not been well-explored. Recently novel organization of respiratory machinery, supercomplex which contains multiple respiratory complexes has been identified. In this study, combing pulse-chase labeling with 2 D Blue Native PAGE (BN-PAGE) analysis6, we carefully investigated the assembly and turn-over of individual complexes and supercomplexes of respiratory chain in various mammalian cell systems. Among the electron transfer chain system, contrarily to previous reported, the biggest complex I is the first to be assembled, in the form of precursor, while complex III is the rate-limiting component of the supercomplexes, which almost co-assembles with the supercomplexes. Our results also demonstrate that while other complexes are relatively stable by themselves, respiratory complex I mostly exists as part of supercomplexes, and the supercomplexes I1 III2, and I1III2IV1 are also more stable than their components. These results indicate that there is common machinery for respiratory complexes assembly, and supercomplexes are the major functional respirasomes.
