报告题目：Supported Lipid Membranes as Biosensing Interface for Label-free SPR Study of Protein Interactions
报告人：Quan Cheng, Professor, Department of Chemistry, University of California, Riverside, CA
主持人：李晓涛 教授
报告时间：9月16日 14:00-15:00（周一）
报告人简介：Quan Cheng博士1986年获得南京大学化学专业本科学位，1989年获得南京大学无机化学硕士学位，1995年获得美国Florida大学分析化学博士学位，之后在美国Univ. of California Berkeley做博士后研究，1997年至2001年间在Lawrence Berkeley National Laboratory任Staff Scientist and Group Leader，2001年至2010年先后任Univ. of California, Riverside化学系助理教授、副教授，2010年提升为正教授。
报告摘要：Biomimetic supported lipid membranes (SLMs) preserve many biophysical properties of cellular membranes and offer a unique system for protein structurefunction study. One major challenge in using SLMs as interface for label-free surface plasmon resonance (SPR) method is the ability to balance functionality and structural robustness. This seminar will discuss the fabrication of SLMs on a novel calcinated nanofilm substrate that proves a highly effective surface for establishing biomimetic interface for affinity study. Specifically, nanoscale silicate layers are built up with layerby-layer (LbL) deposition of poly(allylamine hydrochloride) and sodium silicate on Au, followed by calcination at high temperature to remove the organic component. The approach generates a glass-like thin-film coating, with the thickness ranging from 2 to 20 nm. The nanofilm demonstrates outstanding stability in flow cell conditions and allows for characterization of various membrane-based interactions by SPR methods. Photolithographic techniques can be applied to pattern the silica layers, generating 3D microarrays for analysis with SPR imaging method. The development of SPR imaging (SPRi) technology for monitoring membrane formation process and the application in probing membrane-targeting protein toxins will be presented. In addition, the fundamental aspect of SPR imaging and latest development in instrumentation towards high performance measurement will be covered. Applications of the calcinated nanofilm in membrane stability assessment, carbohydrate-protein interactions, signal amplification towards ultrasensitive detection, and in situ construction of polymeric coating mimicking cell-wall structure using host-guest reactions will be presented.
