活动时间

2007年6月27日

上午：8:30-9:30

活动地点

理科大楼A510

参加人数

40左右

活动名称或主题

 Involvement of the SWI/SNF chromatin remodeling complex in UV damage response

紫外损伤过程中染色体结构重建复合体SWI/SNF的功能

参加人涉及专业

植物学和相关分子生物学研究生、本科生等

活动类型（打√）：  （√）校级报告会    （） 学术沙龙      

活动简要介绍

主讲人               

Dr. Feng Gong（宫锋）：
Assistant Professor, University of Miami Medical School, Miami, FL

个人简历：

1995-1998年  中国科学院上海植物生理生态研究所,微生物分子生物学博士；

1998-2001  美国Stanford University, 博士后；

2001-2003  美国Stanford University, Research Associate；

2003-2007  Research Assistant Professor, Washington State University；

2007-present  Assistant Professor, University of Miami Medical School, Miami, FL

研究领域：细胞周期调控机理和染色体构象调控机理。在Science和Nature等知名刊物上发表论文多篇。

主要研究内容：利用分子生物学方法研究细胞周期调控和染色体修复机制。

代表论文：

1.        F. Gong, D. Fahy and M. Smerdon. 2006. Rad4�CRad23 interaction with SWI/SNF links ATP-dependent chromatin remodeling with nucleotide excision repair. Nature Structural & Molecular Biology 13: 902 - 907 (FG is a corresponding author)

2.        F. Gong, D. Fahy and M.J. Smerdon. 2006. Combination of chemical cross-linking and pull-down assay to study transient protein-protein interactions. Nature Protocols NETWORK. (http://www.natureprotocols.com/2006/10/02 /combination_of_chemical_crossl.php)

3.        F. Gong, Y Kwon and M.J. Smerdon. 2005. Nucleotide excision repair in chromatin and the right of entry. DNA Repair 4(8): 884-96.

4.        M. Gong, F. Gong and C. Yanofsky 2005. Overexpression of tnaC of E. coli inhibits growth by depleting tRNA2Proavailability. J Bacteriol. 188(5): 1892-8.

5.        F. Gong and C. Yanofsky. 2003. Rho’s role in transcription attenuation of the tna operon in E. coli. Methods in Enzymology, 371: 383-91.

6.        F. Gong and C. Yanofsky. 2003. A transcriptional pause synchronizes translation with transcription in the tryptophanase operon leader region. J Bacteriol. 185(21): 6472-6.

7.        F. Gong and C. Yanofsky. 2002. Instruction of translating ribosome by nascent peptide. Science, 297: 1864-7.

8.        F. Gong and C. Yanofsky. 2002. Analysis of tryptophanase operon expression in vitro: accumulation of TnaCpeptidyl- tRNA in S-30 extracts depleted of Release Factor 2 prevents Rho-factor action, simulating induction. J. Biol. Chem, 277(19): 17095-100.

9.        F. Gong, K. Ito, Y. Nakamura and C. Yanofsky. 2001. Proc. Natl. Acad. Sci. USA, 98(16): 8997-9001.

F. Gong and C. Yanofsky. 2001. Reproducing tna operon regulation in vitro in an S-30 system: tryptophan induction inhibits cleavage of TnaC-tRNA. J. Biol. Chem. 276(3): 1974-83.

讲座题目：Involvement of the SWI/SNF chromatin remodeling complex in UV damage response

DNA在受到伤害时如何修复是维持正常生命的重要保证，如果修复不能有效进行，细胞将会发生癌变等，该报告主要是如何利用分子生物学方法阐明在紫外伤害反应过程中，染色体结构重建复合体SWI/SNF发挥的功能。